• Genetic Characterization of Feral Swine in Great Smoky Mountain National Park.

    Metadata Updated: March 17, 2021

    Field Methods: As a component of feral swine control measures conducted by National Park Service employees within Great Smoky Mountain National Park, I would request that either a small piece of ear tissue be collected or approximately 45 hairs be plucked from euthanized hogs (tissue type to be determined by NPS biologists). Specifically for the collection of ear tissue, standard livestock ear notchers will be provided along with cryovials filled with silica beads. The biologist would use the notches to clip a small piece of ear (~1/4”) from the animal. Tweezers or needle nose pliers can be helpful for transferring the tissue from the notchers to the cryovial. Upon insertion of the tissue into the cryovial, desiccation is used to preserve the tissue until genetic analysis can be completed. Alcohol prep wipes are effective for wiping away residual cells, blood, or hair from the cutting service of the notchers before the notchers are used sample another hog. Alternatively, biologists could collect hair from feral swine. Specifically, following euthanasia, pliers can be used to pluck (collection of the hair follicle is essential) approximately 45 hairs from the carcass (generally takes 2 pulls with pliers to get a sufficient number of hairs). The hairs would be transferred to collection cards (small book, approximately the size of a business card with light adhesive to retain hair samples) and the collection card placed in a coin envelop (cards and envelops will be provided).

    Samples in either cryovials or hair cards can be stored at room temperature, in a refrigerator, or frozen; I only ask that collected tissue be removed from field vehicles to avoid the dramatic fluctuations in temperature that can occur. I ask that you record very basic information on the animals collected, specifically sex, age (adult, subadult, juvenile), date of collection, and provide a collection location (I will provide a data sheet). I will provide barcodes stickers that can be placed on either on the hair envelopes or cyrovials as well as the data sheet so that I can match a tissue sample (and subsequent genotype) back to the associated field data. For details on research methods please see attached proposal.

    Access & Use Information

    Public: This dataset is intended for public access and use. License: No license information was provided.

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    Metadata Created Date March 17, 2021
    Metadata Updated Date March 17, 2021

    Metadata Source

    Harvested from NPS-Harvest - NRSS Data Store

    Additional Metadata

    Resource Type Dataset
    Metadata Created Date March 17, 2021
    Metadata Updated Date March 17, 2021
    Publisher National Park Service
    Unique Identifier Unknown
    Identifier NPS_DataStore_2258961
    Data First Published 2019-02-08
    Data Last Modified 2019-02-08
    Category Generic Dataset
    Public Access Level public
    Bureau Code 010:24
    Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
    Metadata Catalog ID D:\IRMA\DataStore\Application\OpenData\v1.1\NPS-DataStore.json
    Schema Version https://project-open-data.cio.gov/v1.1/schema
    Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
    Data Quality True
    Harvest Object Id 8116f8eb-4793-406e-94b0-7a4a159c71e2
    Harvest Source Id a73e9b95-126c-4791-a8f2-46fc064cdc62
    Harvest Source Title NPS-Harvest - NRSS Data Store
    Homepage URL https://irma.nps.gov/DataStore/Reference/Profile/2258961
    Program Code 010:118, 010:119
    Related Documents https://irma.nps.gov/DataStore/Reference/Profile/2258961
    Source Datajson Identifier True
    Source Hash 4e1cc4d45234faa679c15602555e643923dc35da
    Source Schema Version 1.1
    Spatial -84.01389,35.4269066,-82.99805,35.8414
    Temporal 2014-04-03/2018-10-24

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